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Development of a duplex real time PCR for the detection of<i>Rickettsia</i>spp. and typhus group rickettsia in clinical samples

21 Nov 2011

Abstract excerpt

Molecular diagnosis using real-time polymerase chain reaction (PCR) may allow earlier diagnosis of rickettsiosis. We developed a duplex real-time PCR that amplifies (1) DNA of any rickettsial species and (2) DNA of both typhus group rickettsia, that is, Rickettsia prowazekii and Rickettsia typhi. Primers and probes were selected to amplify a segment of the 16S rRNA gene of Rickettsia spp. for the pan-rickettsial...

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PMID: 22098502
DOI: 10.1111/j.1574-695x.2011.00910.x

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