Article
Construction of a new catabolic pathway for D-fructose in Escherichia coli K12 using an L-sorbose-specific enzyme from Klebsiella pneumoniae.
Archives of microbiology - 1 Jan 1990
Wöhrl B M, Sprenger G A, Lengeler J W
Abstract excerpt
Starting with a fruK (formerly fpk) mutant of Escherichia coli K12 lacking D-fructose-1-phosphate kinase (E.C. 2.7.1.3.), fructose positive derivatives were isolated after introduction of the cloned gene sorE from Klebsiella pneumoniae coding for an L-sorbose-1-phosphate reductase. The new pathway was shwon to proceed from D-fructose via D-fructose-1-phosphate and D-mannitol-1-phosphate to D-fructose 6-phosphate....
Topics
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Fructose
- Klebsiella pneumoniae
- Mutation
- Operon
- R Factors
- Restriction Mapping
- Sorbose
- Sugar Alcohol Dehydrogenases