Article
Direct selection for mutants with increased K+ transport in Saccharomyces cerevisiae.
Genetics - 1 Jun 1990
Vidal M, Buckley A M, Hilger F, Gaber R F
Abstract excerpt
Saccharomyces cerevisiae cells containing a deletion of TRK1, the gene encoding the high affinity potassium transporter, retain only low affinity uptake of this ion and consequently lose the ability to grow in media containing low levels (0.2 mM) of potassium. Using a trk1 delta strain, we selected spontaneous Trk+ pseudorevertants that regained the ability to grow on low concentrations of potassium. The...
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