Article
Complementation of Escherichia coli sigma 54 (NtrA)-dependent formate hydrogenlyase activity by a cloned Thiobacillus ferrooxidans ntrA gene.
Journal of bacteriology - 1 Aug 1990
Berger D K, Woods D R, Rawlings D E
Abstract excerpt
The ntrA gene of Thiobacillus ferrooxidans was cloned by complementation of an Escherichia coli ntrA mutant that was unable to produce gas via the sigma 54 (NtrA)-dependent formate hydrogenlyase pathway. Analysis of the DNA sequence showed that the T. ferrooxidans ntrA gene coded for a protein of...
Topics
- Acidithiobacillus thiooxidans
- Aldehyde Oxidoreductases
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Formate Dehydrogenases
- Genes, Bacterial
- Genetic Complementation Test
- Genotype
- Hydrogenase
- Molecular Sequence Data
- Multienzyme Complexes
- Mutation
- Plasmids
- Protein Biosynthesis
- Restriction Mapping