Article
Direct estimation of serological H-Y antigen by flow cytometry.
Human genetics - 1 Jun 1990
Kent M, Wachtel S, Thaler H T
Abstract excerpt
This study was undertaken to evaluate the efficiency of flow cytometry in the detection of the serological H-Y antigen, and to survey expression of H-Y in the normal human population. Peripheral blood leukocytes (granulocytes) were reacted with monoclonal H-Y antibody, gw-16, and with FITC-conjugated goat anti-mouse IgG, and then were scored for fluorescence in the flow cytometer. Assigned H-Y phenotype was...
Topics
- Antibodies, Monoclonal
- Female
- Flow Cytometry
- Fluorescent Antibody Technique
- Granulocytes
- H-Y Antigen
- Humans
- Male
- Phenotype
- Predictive Value of Tests
- Reproducibility of Results
- Sex Determination Analysis