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Article

Molecular identification of Penicillium marneffei using rolling circle amplification

19 Sept 2011

Abstract excerpt

Penicillium marneffei is the aetiological agent of a severe systemic disease in immunocompromised hosts in Southeast Asia. In the present study, we evaluated an identification method based on rolling circle amplification (RCA) enabling rapid and specific detection of single nucleotide differences. Three padlock probes were designed on the basis of the internal transcribed spacers 1 and 2 (ITS) of the rRNA operon....

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PMID: 21929692
DOI: 10.1111/j.1439-0507.2011.02017.x

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