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Article

Rapid identification of fungal pathogens by rolling circle amplification using Fonsecaea as a model

1 Sept 2011

Abstract excerpt

We aimed to describe a rapid and sensitive assay for identification of pathogenic fungi without sequencing. The method of rolling circle amplification (RCA) is presented with species of Fonsecaea, agents of human chromoblastomycosis, as a model. The internal transcribed spacer (ITS) rDNA region of 103 Fonsecaea strains was sequenced and aligned in view of designing three specific padlock probes to be used for the...

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PMID: 21910759
DOI: 10.1111/j.1439-0507.2010.01995.x

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