Article
Cloning and sequence of two different cDNAs encoding 1-aminocyclopropane-1-carboxylate synthase in tomato.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jun 1990
Van der Straeten D, Van Wiemeersch L, Goodman H M, Van Montagu M
Abstract excerpt
1-Aminocyclopropane-1-carboxylate synthase (ACC synthase; S-adenosyl-L-methionine methylthioadenosine-lyase, EC 4.4.1.14), the key enzyme in ethylene biosynthesis, was purified 5000-fold from induced tomato pericarp. ACC synthase activity was unambiguously correlated with a 45-kDa protein by two...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA
- Escherichia coli
- Gene Library
- Isoenzymes
- Kinetics
- Lyases
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- Peptide Mapping
- Plants
- Polymerase Chain Reaction
- Recombinant Proteins
- Restriction Mapping