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Detection of <i>Anaplasma phagocytophilum</i> in Brazilian dogs by real-time polymerase chain reaction

15 Jun 2011

Abstract excerpt

Anaplasma phagocytophilum was detected in dogs from Brazil in the municipalities of Seropédica and Itaguaí, Rio de Janeiro state, by real-time polymerase chain reaction (PCR) using SYBR Green to detect the amplification. Of 253 samples, 18 (7.11%) were positive, with a threshold cycle (Ct) ranging from 31 to 35 cycles. The PCR product from a positive sample was cloned and sequenced. The sequence obtained...

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PMID: 21908321
DOI: 10.1177/1040638711406974

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