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Duplex Real-Time Polymerase Chain Reaction for Simultaneous Detection and Quantification of <i>Anaplasma Marginale</i> and <i>Anaplasma Centrale</i>

1 Sept 2008

Abstract excerpt

Anaplasma marginale and Anaplasma centrale are rickettsial pathogens responsible for acute disease and mild infections, respectively, in cattle herds. A duplex real-time polymerase chain reaction (PCR) assay with probes labeled with different fluorophores was developed for simultaneous detection and quantification of A. marginale and A. centrale DNA in bovine blood samples. The assay was able to detect as few as...

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PMID: 18776093
DOI: 10.1177/104063870802000511

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