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Detection of invasive infection caused byFusarium solaniand non-Fusarium solanispecies using a duplex quantitative PCR-based assay in a murine model of fusariosis

1 Apr 2012

Abstract excerpt

A duplex Real Time PCR (RT-PCR) assay for detecting DNA of members of the genus Fusarium has been developed and validated by using two mouse models of invasive infection. The duplex RT-PCR technique employed two specific molecular beacon probes targeting a highly conserved region of the fungal rDNA gene. This technique showed a detection limit of 10 fg DNA per μl of sample and a specificity of 100%. The...

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PMID: 21905946
DOI: 10.3109/13693786.2011.604047

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