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Cloning, characterization and diversity of insecticidal crystal protein genes of bacillus thuringiensis native isolates from soils of Andaman and Nicobar Islands.

Current microbiology - 1 Nov 2011

Mahadeva Swamy H M, Asokan R, Nagesha S N, Arora D K, Birah Ajantha, Mahmood Riaz

Abstract excerpt

Bt strains were isolated from soils of Andaman and Nicobar Islands and characterized by microscopic and molecular methods. Diversity was observed both in protein and cry gene profiles, where majority of the isolates showed presence of 65 kDa protein band on SDS-PAGE while rest of them showed 130, 72, 44, and 29 kDa bands. PCR analysis revealed predominance of cry1I and cry7, 8 genes in these isolates. The PCR...

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PMID: 21858696
DOI: 10.1007/s00284-011-9998-x

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