Article
Oligonucleotide microarray expression profiling of contrasting invasive phenotypes in colorectal cancer.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Thorn Christopher C, Williams Deborah, Freeman Thomas C
Abstract excerpt
This chapter refers to the application of laser-capture microdissection with oligonucleotide microarray analysis. The protocol described has been successfully used to identify differential transcript expression between contrasting colorectal cancer invasive phenotypes. Tissue processing, RNA extraction, quality control, amplification, fluorescent labelling, purification, hybridisation, and elements of data...
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