Article
Excision of the En/Spm transposable element of Zea mays requires two element-encoded proteins.
The EMBO journal - 1 Dec 1990
Frey M, Reinecke J, Grant S, Saedler H, Gierl A
Abstract excerpt
An excision assay system for En/Spm was developed in transgenic tobacco. The characteristics of excision and integration are similar to the natural system of Zea mays. In this transgenic model system two En/Spm encoded trans-acting functions, TNPA and TNPD, are required for excision. A biochemica...
Topics
- Cloning, Molecular
- Crosses, Genetic
- DNA Transposable Elements
- DNA-Binding Proteins
- Molecular Sequence Data
- Oligonucleotide Probes
- Phenotype
- Plant Proteins
- Plants, Toxic
- Plasmids
- Polymerase Chain Reaction
- Promoter Regions, Genetic
- Repressor Proteins
- Restriction Mapping
- Nicotiana
- Transcription, Genetic
- Zea mays