Article
Development of real-time PCR assay for differential detection and quantification for multiple Babesia microti-genotypes.
Parasitology international - 1 Dec 2011
Ohmori Shiho, Kawai Atsuko, Takada Nobuhiro, Saito-Ito Atsuko
Abstract excerpt
We have developed a real-time PCR assay that can rapidly and differentially detect and quantify four genotypes of small subunit ribosomal RNA gene (SSUrDNA) of Babesia microti (Kobe-, Otsu-, Nagano- and US-types). In this assay, four genotype-specific pairs of primers targeted on internal transcribed spacer (ITS) 1 or 2 sequences were used and amplicons by each pair of primers were quantitatively detected by...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.