Article
Detection of toxic lignin hydrolysate-related compounds using an inaA::luxCDABE fusion strain.
Journal of biotechnology - 20 Feb 2012
Lee Siseon, Mitchell Robert J
Abstract excerpt
Real-time quantitative PCR analyses of Escherichia coli str. BL21(DE3) exposed to 0.5 g/L ferulic and coumaric acid showed that the inaA gene was significantly induced (7.7- and 3.6-fold higher, respectively). Consequently, a transcriptional fusion of the inaA promoter with the luxCDABE operon was constructed and characterized with several compounds identified within hydrolysates. Tests demonstrated that the...
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