Article
Identification and expression of the Escherichia coli fdhD and fdhE genes, which are involved in the formation of respiratory formate dehydrogenase.
Journal of bacteriology - 1 Oct 1990
Schlindwein C, Giordano G, Santini C L, Mandrand M A
Abstract excerpt
The two closely linked fdhD and fdhE genes of Escherichia coli are required for the formation of active membrane-bound phenazine methosulfate-linked formate dehydrogenase (FDH-PMS). Both genes were isolated from a cosmid library. Restriction endonuclease analysis associated with Mu dII1734 insertion mutagenesis indicated that the two genes were separated by at least 4 kilobases and transcribed in opposite...
Topics
- Cloning, Molecular
- DNA Transposable Elements
- DNA-Directed RNA Polymerases
- Escherichia coli
- Formate Dehydrogenases
- Gene Expression
- Genes, Bacterial
- Genotype
- Mutation
- Oxygen Consumption
