Article
Transposition of Tn916 to different sites in the chromosome of Neisseria meningitidis: a genetic tool for meningococcal mutagenesis.
Molecular microbiology - 1 May 1990
Kathariou S, Stephens D S, Spellman P, Morse S A
Abstract excerpt
The difficulty in obtaining mutants in pathogenic Neisseria has limited the ability to genetically define determinants responsible for virulence as well as the ability to generate a genetic map. We show that the 16.5kb conjugative transposon Tn916 can be introduced into Neisseria meningitidis on the suicide vectors pAM120 and pAM170. After introduction, Tn916 transposed to different sites in the chromosome of...
Topics
- Chromosomes
- DNA Transposable Elements
- DNA, Bacterial
- Drug Resistance, Microbial
- Mutation
- Neisseria meningitidis
- Plasmids
- Transformation, Bacterial