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Rapid and reliable high‐throughput methods of DNA extraction for use in barcoding and molecular systematics of mushrooms

30 Dec 2009

Abstract excerpt

We present two methods for DNA extraction from fresh and dried mushrooms that are adaptable to high-throughput sequencing initiatives, such as DNA barcoding. Our results show that these protocols yield ∼85% sequencing success from recently collected materials. Tests with both recent (<2 year) and older (>100 years) specimens reveal that older collections have low success rates and may be an inefficient resource...

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PMID: 21565067
DOI: 10.1111/j.1755-0998.2009.02825.x

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