Article
Purification of flavanone 3 beta-hydroxylase from Petunia hybrida: antibody preparation and characterization of a chemogenetically defined mutant.
Archives of biochemistry and biophysics - 1 Feb 1990
Britsch L
Abstract excerpt
Flavanone 3 beta-hydroxylase from Petunia hybrida has been purified to apparent homogeneity utilizing an improved purification protocol including chromatography of the partially purified enzyme on hydroxyapatite, chromatofocusing, and hydrophobic interaction chromatography on phenyl-Superose. The specificity of mouse and rabbit polyclonal antisera directed to flavanone 3 beta-hydroxylase was demonstrated by...
Topics
- Antigen-Antibody Complex
- Blotting, Western
- Chromatography
- Chromatography, Gel
- Chromatography, Ion Exchange
- Durapatite
- Hydroxyapatites
- Immunoassay
- Indicators and Reagents
- Kinetics
- Mixed Function Oxygenases