Article
The Arabidopsis REF8 gene encodes the 3-hydroxylase of phenylpropanoid metabolism.
The Plant journal : for cell and molecular biology - 1 Apr 2002
Franke Rochus, Humphreys John M, Hemm Matthew R, Denault Jeff W, Ruegger Max O, Cusumano Joanne C, Chapple Clint
Abstract excerpt
The activity of p-coumarate 3-hydroxylase (C3H) is thought to be essential for the biosynthesis of lignin and many other phenylpropanoid pathway products in plants; however, no conditions suitable for the unambiguous assay of the enzyme are known. As a result, all attempts to purify the protein and clone its corresponding gene have failed. By screening for plants that accumulate reduced levels of soluble...
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