Article
Nuclear transfer of embryonic cell nuclei to non-enucleated eggs in zebrafish, Danio rerio.
International journal of biological sciences - 15 Apr 2011
Hattori Manabu, Hashimoto Hisashi, Bubenshchikova Ekaterina, Wakamatsu Yuko
Abstract excerpt
We previously established a novel method for nuclear transfer in medaka (Oryzias latipes) using non-enucleated, diploidized eggs as recipients for adult somatic cell nuclei. Here we report the first attempt to apply this method to another fish species. To examine suitability of using non-enucleated eggs as recipients for nuclear transfer in the zebrafish (Danio rerio), we transferred blastula cell nuclei from a...
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