Article
Comparative evaluation of different DNA extraction methods for HPV genotyping by linear array and INNO-LiPA.
Journal of medical virology - 1 Jun 2011
Donà Maria Gabriella, Benevolo Maria, Pimpinelli Fulvia, Battista Mara, Rollo Francesca, Stivali Francesca, Moscarelli Antonella, Giuliani Massimo, Di Carlo Aldo, Vocaturo Amina
Abstract excerpt
In order to investigate the influence of DNA extraction on two PCR-based HPV genotyping tests (Linear Array, Roche and INNO-LiPA Extra, Innogenetics), three different procedures were used to purify DNA from 28 cervico-vaginal samples tested previously by the Hybrid Capture 2: the AmpliLute Liquid Media Extraction kit (Roche), the QIAamp DNA Blood mini kit (QIAGEN), and the NucliSENS EasyMAG automated platform...
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