Article
Zebrafish embryo development in a microfluidic flow-through system.
Lab on a chip - 21 May 2011
Wielhouwer Eric M, Ali Shaukat, Al-Afandi Abdulrahman, Blom Marko T, Riekerink Marinus B Olde, Poelma Christian, Westerweel Jerry, Oonk Johannes, Vrouwe Elwin X, Buesink Wilfred, vanMil Harald G J, Chicken Jonathan, van't Oever Ronny, Richardson Michael K
Abstract excerpt
The zebrafish embryo is a small, cheap, whole-animal model which may replace rodents in some areas of research. Unfortunately, zebrafish embryos are commonly cultured in microtitre plates using cell-culture protocols with static buffer replacement. Such protocols are highly invasive, consume large quantities of reagents and do not readily permit high-quality imaging. Zebrafish and rodent embryos have previously...
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