Article
Detection and mapping of mtDNA SNPs in Atlantic salmon using high throughput DNA sequencing.
BMC genomics - 7 Apr 2011
Fridjonsson Olafur, Olafsson Kristinn, Tompsett Scott, Bjornsdottir Snaedis, Consuegra Sonia, Knox David, de Leaniz Carlos Garcia, Magnusdottir Steinunn, Olafsdottir Gudbjorg, Verspoor Eric, Hjorleifsdottir Sigridur
Abstract excerpt
BACKGROUND: Approximately half of the mitochondrial genome inherent within 546 individual Atlantic salmon (Salmo salar) derived from across the species' North Atlantic range, was selectively amplified with a novel combination of standard PCR and pyro-sequencing in a single run using 454 Titanium FLX technology (Roche, 454 Life Sciences). A unique combination of barcoded primers and a partitioned sequencing plate...
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