Alcohol treatment of defective lambda lysogens is deletionogenic.

Abstract excerpt

We ascertained that transient exposure to ethanol, above 18%, was deletionogenic to an Escherichia coli strain with a fragment (12.5 kb) of bacteriophage lambda integrated within the chromosome. The lambda attL B.P' through P fragment provided a forward selection for mutants, and a target for mutagenesis. The cells were killed by thermal derepression of transcription and replication of the lambda fragment when...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Bacteriophage lambda
• Share to Blotting, Southern
• Share to Chromosome Deletion