Article
Alcohol treatment of defective lambda lysogens is deletionogenic.
Molecular & general genetics : MGG - 1 Jun 1990
Hayes S, Duncan D, Hayes C
Abstract excerpt
We ascertained that transient exposure to ethanol, above 18%, was deletionogenic to an Escherichia coli strain with a fragment (12.5 kb) of bacteriophage lambda integrated within the chromosome. The lambda attL B.P' through P fragment provided a forward selection for mutants, and a target for mut...
Topics
- Bacteriophage lambda
- Blotting, Southern
- Chromosome Deletion
- DNA, Viral
- Escherichia coli
- Ethanol
- Lysogeny
- Mutation
- Phenotype
