Article
[Developing transgenic barley lines producing human lactoferrin using mutant alfa-tubulin gene as selective marker gene].
TSitologiia i genetika - 1 Jan 2000
Tanasienko I V, Emets A I, Pirko Ia V, Korkhovoĭ V I, Abumkhadi N, Blium Ia B
Abstract excerpt
Method of biolistic transformation was used for genetic improvement of commercial barley cultivars (Oksamitoviy, Vodogray and Getman). The plasmid pHLFTuBA was used for particle bombardment that consists of the hLF gene under the control of the barley glutelin B-1 promoter and a selectable marker gene, alpha-tubulin conferring resistance to trifluralin (dinitroanilinr herbicide). Preliminary screening of...
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