Article
Identification of recombinant intermediates of hepatitis B virus between genotype B and C in vitro.
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences - 1 Feb 2011
Chen Junyi, Huang Ailong, Xu Li, Chen Dianquan, Yu Hong, Zhu Zhaojing, Huang Zuchun, Yang Zongfa, Chen Lishu, Tan Tao
Abstract excerpt
OBJECTIVE: To detect the recombinant intermediates of hepatitis B virus (HBV) between genotype B and C in vitro. METHODS: Vector Plenti6/V5-D-topo-X was genetically modified by genotype B and C to transfect HepG2 cells. Then the HepG2 cells were amplified and sequence of the nucleic acid after the transinfection was tested and compared with RDP3Beta40 software package and bootscanning procedure in SimPlot program...
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