Article
[Ethylmethane sulfonate induction of auxotrophic mutants of Rhizobium meliloti and their characteristics].
Mikrobiologiia - 1 Jan 2000
Fedorov S N, Zaretskaia A N
Abstract excerpt
Ethylmethanesulfonate was used as a mutagen to induce auxotrophic mutants in Rhizobium meliloti L5-30. Survival of the culture depended on the period of time during which it was treated with the mutagen. Thirteen auxotrophic mutants were isolated as a result of screening 1404 clones, and their requirements in growtn factors were determined. Ethylmethanesulfonate induced mainly those mutants which required...
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