[Ethylmethane sulfonate induction of auxotrophic mutants of Rhizobium meliloti and their characteristics].

Article

[Ethylmethane sulfonate induction of auxotrophic mutants of Rhizobium meliloti and their characteristics].

Mikrobiologiia - 1 Jan 2000

Fedorov S N, Zaretskaia A N

Abstract excerpt

Ethylmethanesulfonate was used as a mutagen to induce auxotrophic mutants in Rhizobium meliloti L5-30. Survival of the culture depended on the period of time during which it was treated with the mutagen. Thirteen auxotrophic mutants were isolated as a result of screening 1404 clones, and their re...

Topics

Ethyl Methanesulfonate
Mesylates
Mutation
Nitrogen Fixation
Rhizobium
Symbiosis
Time Factors

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