Article
A novel rhodobacter sphaeroides expression system for real-time evaluation of heterologous protein expression levels.
Protein and peptide letters - 1 Jun 2011
Zhao Zhiping, Hu Zongli, Nie Xin, Cheng Lijing, Ding Guolin, Luo Min, Pan Yu, Liang Yan, Chen Guoping
Abstract excerpt
Heterologous protein expression levels can not be evaluated in real-time by experimental procedures commonly used for most expression systems during host cell culture. Rb. sphaeroides has provided an ideal system for studying both photosynthesis and membrane development and exhibited potential as a novel expression system. We constructed the puc1BA and puc2BA mutant strain Rb. sphaeroides CQU68 and used it as a...
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