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Specific detection of Mycobacterium tuberculosis complex strains by polymerase chain reaction

1 Jun 1990

Abstract excerpt

During the screening of a Mycobacterium tuberculosis lambda gt-11 gene library with monoclonal antibodies, we detected a recombinant clone, lambda PH7311, which contained a mycobacterial DNA insert that hybridized specifically with DNA of M. tuberculosis complex strains. Part of this insert was sequenced and used for the development of an M. tuberculosis complex-specific polymerase chain reaction (PCR). Only...

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PMID: 2116445
DOI: 10.1128/jcm.28.6.1204-1213.1990

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