Article
Purification and characterization of glycogen synthase from a glycogen-deficient strain of Saccharomyces cerevisiae.
The Journal of biological chemistry - 15 Aug 1990
Peng Z Y, Trumbly R J, Reimann E M
Abstract excerpt
Chromatography of wild-type yeast extracts on DEAE-cellulose columns resolves two populations of glycogen synthase I (glucose-6-P-independent) and D (glucose-6-P-dependent) (Huang, K. P., Cabib, E. (1974) J. Biol. Chem. 249, 3851-3857). Extracts from a glycogen-deficient mutant strain, 22R1 (glc7), yielded only the D form of glycogen synthase. Glycogen synthase D purified from either wild-type yeast or from this...
Topics
- Chromatography, DEAE-Cellulose
- Chromatography, High Pressure Liquid
- Electrophoresis, Polyacrylamide Gel
- Glycogen
- Glycogen Synthase
- Immunoblotting
- Isoenzymes
- Kinetics
- Molecular Weight
- Mutation
- Peptide Mapping
