Lysine can replace arginine 67 in the mediation of covalent attachment of FAD to histidine 71 of 6-hydroxy-D-nicotine oxidase.

Article

Lysine can replace arginine 67 in the mediation of covalent attachment of FAD to histidine 71 of 6-hydroxy-D-nicotine oxidase.

The Journal of biological chemistry - 5 Aug 1990

Mauch L, Bichler V, Brandsch R

Abstract excerpt

The requirements for FAD-attachment to His71 of 6-hydroxy-D-nicotine oxidase (6-HDNO) were investigated by site-directed mutagenesis. The following amino acid replacements were introduced into the sequence Arg67-Ser68-Gly69-Gly70-His71 of the 6-HDNO-polypeptide: 1) Arg67 was replaced with Ala (A1...

Topics

Amino Acid Sequence
Arginine
Binding Sites
Escherichia coli
Flavin-Adenine Dinucleotide
Histidine
Lysine
Molecular Sequence Data
Mutation
Oligonucleotide Probes
Oxidoreductases
Plasmids

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