Comparison of four methods, including semi-automated rep-PCR, for the typing of vancomycin-resistant Enterococcus faecium.

doi:10.1016/j.mimet.2010.10.014

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Article

Comparison of four methods, including semi-automated rep-PCR, for the typing of vancomycin-resistant Enterococcus faecium.

Journal of microbiological methods - 1 Jan 2011

Bourdon Nancy, Lemire Astrid, Fines-Guyon Marguerite, Auzou Michel, Périchon Bruno, Courvalin Patrice, Cattoir Vincent, Leclercq Roland

Abstract excerpt

We have assessed the performance of semi-automated rep-PCR (Diversilab®) and multilocus sequence typing (MLST) in comparison to pulsed-field gel electrophoresis (PFGE) for typing a collection of 29 epidemiologically characterized vancomycin-resistant Enterococcus faecium (VRE). Sixteen strains that harbored the Tn1546 element were typed by PCR mapping. The discriminative power of the typing methods was calculated...

Topics

Automation
Bacterial Typing Techniques
DNA Transposable Elements
DNA, Bacterial
Electrophoresis, Gel, Pulsed-Field
Enterococcus faecium
Genetic Variation
Genotype
Gram-Positive Bacterial Infections

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Humans

Molecular Typing

Multilocus Sequence Typing

Polymerase Chain Reaction

Reproducibility of Results

Vancomycin Resistance

Source

Open in PubMed

Identifiers

PMID: 21062634
DOI: 10.1016/j.mimet.2010.10.014

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-21062634-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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