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Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region

1 Sept 2010

Abstract excerpt

We present filaria-nested polymerase chain reaction (PCR), which is based on amplification of first internal transcribed spacer rDNA to distinguish three parasitic filarial species (Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans) that can be found in the Amazon Region. Nested PCR-based identifications yielded the same results as those utilizing morphological characters. Nested PCR is highly...

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Insect symbiosis and bacterial influences
Parasite Biology and Host Interactions
Parasitic Diseases Research and Treatment

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 20945000
DOI: 10.1590/s0074-02762010000600016

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