Article
Centrifugal microfluidic system for primary amplification and secondary real-time PCR.
Lab on a chip - 7 Dec 2010
Focke Maximilian, Stumpf Fabian, Roth Günter, Zengerle Roland, von Stetten Felix
Abstract excerpt
Pre-amplification is a basis for numerous polymerase chain reaction (PCR) protocols but bears severe contamination risks due to handling of high-copy DNA samples. Therefore we developed a self-contained centrifugal microfluidic system comprising pre-stored reagents; it enables pre-amplification o...
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