Article
Detection of Mycobacterium leprae DNA from archaeological skeletal remains in Japan using whole genome amplification and polymerase chain reaction.
PloS one - 26 Aug 2010
Suzuki Koichi, Takigawa Wataru, Tanigawa Kazunari, Nakamura Kazuaki, Ishido Yuko, Kawashima Akira, Wu Huhehasi, Akama Takeshi, Sue Mariko, Yoshihara Aya, Mori Shuichi, Ishii Norihisa
Abstract excerpt
BACKGROUND: Identification of pathogen DNA from archaeological human remains is a powerful tool in demonstrating that the infectious disease existed in the past. However, it is very difficult to detect trace amounts of DNA remnants attached to the human skeleton, especially from those buried in a humid atmosphere with a relatively high environmental temperature such as in Asia. METHODOLOGY/PRINCIPAL FINDINGS:...
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