Two New Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction Assays with Unique Target Sites for the Specific and Sensitive Detection of Lineages 1 and 2 <i>West Nile Virus</i> Strains

Abstract excerpt

Two novel 1-step real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays for the simultaneous detection of West Nile virus (WNV) lineage 1 and 2 strains were developed. Primers and the probe of assay 1 target the 5'-untranslated region (UTR), whereas the amplicon of assay 2 is located in the nonstructural region NS2A, which enables an unambiguous and independent WNV diagnosis based...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Malaria Research and Control
• Share to Mosquito-borne diseases and control
• Share to Viral Infections and Vectors