Article
Combined multiphoton imaging and automated functional enucleation of porcine oocytes using femtosecond laser pulses
1 Jan 2010
Abstract excerpt
Since the birth of "Dolly" as the first mammal cloned from a differentiated cell, somatic cell cloning has been successful in several mammalian species, albeit at low success rates. The highly invasive mechanical enucleation step of a cloning protocol requires sophisticated, expensive equipment and considerable micromanipulation skill. We present a novel noninvasive method for combined oocyte imaging and...
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