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Article

Composition and genetic diversity of picoeukaryotes in subtropical coastal waters as revealed by 454 pyrosequencing.

The ISME journal - 1 Aug 2010

Cheung Man Kit, Au Chun Hang, Chu Ka Hou, Kwan Hoi Shan, Wong Chong Kim

Abstract excerpt

Information on genetic diversity of picoeukaryotes (<2-3 microm) comes mainly from traditional gene cloning and sequencing, but this method suffers from cloning biases and limited throughput. In this study, we explored the feasibility of using the cloning-independent and massively parallel 454 pyrosequencing technology to study the composition and genetic diversity of picoeukaryotes in the coastal waters of the...

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PMID: 20336159
DOI: 10.1038/ismej.2010.26

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