Article
Isolation and characterization of Chlamydomonas temperature-sensitive mutants affecting gametic differentiation under nitrogen-starved conditions.
Current genetics - 1 Feb 1991
Saito T, Matsuda Y
Abstract excerpt
Two conditional mutants of Chlamydomonas reinhardtii, dif-1 and dif-2, affecting gametic differentiation under conditions of nitrogen (N)-starvation, have been isolated. These mutant cell remain "vegetative" at the restrictive temperature (35 degrees C) in -N medium, as defined by assays of cell body-agglutinin and cell wall lytic enzyme activities in the soluble fractions of cell homogenates. Moreover, the...
Topics
- Chlamydomonas
- Diploidy
- Gametogenesis
- Genetic Linkage
- Mutation
- Nitrogen
- Phenotype
- Protein Biosynthesis
- Solubility
- Temperature
