Article
[Screening and genetic diversity analysis of microsatellite markers in Chinese lobster (Panulirus stimpsoni)].
Yi chuan = Hereditas - 1 Jul 2010
Liu Chu-Wu, Li Jin-Ming, Liu Li, Guo Yu-Song
Abstract excerpt
With the construction of a library of partial fractionated genomic DNA of Panulirus stimpsoni Hoehuis, the microsatellite sequences of P. stimpsoni were screened by PCR technique. Then, the genetic diversity was analyzed with the microsatellite markers. Seventy-eight microsatellite sequences in 55 positive recombinant clones were obtained by PCR technique with primers of M13+/- and (CT)15, and (AT)15. Among these...
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