Article
The proline 160 in the selectivity filter of the Arabidopsis NO(3)(-)/H(+) exchanger AtCLCa is essential for nitrate accumulation in planta.
The Plant journal : for cell and molecular biology - 1 Sept 2010
Wege Stefanie, Jossier Mathieu, Filleur Sophie, Thomine Sébastien, Barbier-Brygoo Hélène, Gambale Franco, De Angeli Alexis
Abstract excerpt
Nitrate, the major nitrogen source for plants, can be accumulated in the vacuole. Its transport across the vacuolar membrane is mediated by AtCLCa, an antiporter of the chloride channel (CLC) protein family. In contrast to other CLC family members, AtCLCa transports nitrate coupled to protons. Recently, the different behaviour towards nitrate of CLC proteins has been linked to the presence of a serine or proline...
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