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Detection and Characterization of Human <i>Ureaplasma</i> Species and Serovars by Real-Time PCR

17 Jun 2010

Abstract excerpt

We designed primers and probes for the detection and discrimination of Ureaplasma parvum and U. urealyticum and their 14 serovars by real-time PCR. The analytical sensitivity and specificity of the multiplex species-specific PCR were determined by testing corresponding American Type Culture Collection (ATCC) type strains, 47 other microbial species, and human genomic DNA. The limits of the multiplex PCR were 2.8...

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PMID: 20554828
DOI: 10.1128/jcm.01877-09

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