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Article

A combined RT-PCR and dot-blot hybridization method reveals the coexistence of SJNNV and RGNNV betanodavirus genotypes in wild meagre (Argyrosomus regius).

Journal of applied microbiology - 1 Oct 2010

Lopez-Jimena B, Cherif N, Garcia-Rosado E, Infante C, Cano I, Castro D, Hammami S, Borrego J J, Alonso M C

Abstract excerpt

AIMS: To detect the possible coexistence of striped jack nervous necrosis virus (SJNNV) and red-spotted grouper nervous necrosis virus (RGNNV) genotypes in a single fish, a methodology based on the combination of PCR amplification and blot hybridization has been developed and applied in this study. METHODS AND RESULTS: The degenerate primers designed for the PCR procedure target the T4 region within the capsid...

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PMID: 20497277
DOI: 10.1111/j.1365-2672.2010.04759.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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