Article
Excess production of Bacillus subtilis quercetin 2,3-dioxygenase affects cell viability in the presence of quercetin.
Bioscience, biotechnology, and biochemistry - 1 Jan 2010
Hirooka Kazutake, Fujita Yasutaro
Abstract excerpt
Bacillus subtilis quercetin 2,3-dioxygenase (QdoI) catalyzes the C-ring cleavage of quercetin to yield 2-protocatechuoyl-phloroglucinol carboxylic acid and carbon monoxide. The recombinant QdoI effectively decomposed several flavonols, including quercetin, whereas its activity toward fisetin was low, suggesting that the 5-hydroxyl group at the A-ring is critical for substrate recognition. A B. subtilis mutant...
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