Article
Validation of half-reaction amplification using Promega PowerPlex 16.
Journal of forensic sciences - 1 Jul 2010
Hoffman Nicole H, Fenger Terry
Abstract excerpt
DNA amplification is a fundamental yet costly process used in DNA analysis. This study evaluated half-reaction amplification (12.5, 12, and 13 microL) using the Promega Powerplex 16 Kit with the hope of reducing sample analysis costs by half. A sensitivity study was completed, along with the testing of various blood stain samples including those with low (<0.40 ng) and high DNA concentrations (>3.0 ng), peak...
Topics
- Alleles
- DNA
- DNA Fingerprinting
- Electrophoresis
- Humans
- Polymerase Chain Reaction
- Tandem Repeat Sequences
