Article
Development of a real-time RT-PCR assay based on primer-probe energy transfer for the detection of all serotypes of bluetongue virus.
Journal of virological methods - 1 Aug 2010
Leblanc N, Rasmussen T B, Fernández J, Sailleau C, Rasmussen L D, Uttenthal A, Zientara S, Belák S, Hakhverdyan M
Abstract excerpt
A real-time RT-PCR assay based on the primer-probe energy transfer (PriProET) was developed to detect all 24 serotypes of bluetongue virus (BTV). BTV causes serious disease, primarily in sheep, but in other ruminants as well. A distinguishing characteristic of the assay is its tolerance toward mutations in the probe region. Furthermore, melting curve analysis following immediately PCR confirms specific probe...
Topics
- Animals
- Bluetongue
- Bluetongue virus
- DNA Primers
- Denmark
- Disease Outbreaks
- Energy Transfer
- Genotype
- Mutation
- Oligonucleotide Probes
- Reverse Transcriptase Polymerase Chain Reaction