Development of real-time RT-qPCR assays for the typing of two novel bluetongue virus genotypes derived from sheeppox vaccine.

doi:10.1016/j.jviromet.2021.114288

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Article

Development of real-time RT-qPCR assays for the typing of two novel bluetongue virus genotypes derived from sheeppox vaccine.

Journal of virological methods - 1 Dec 2021

King Simon, Flannery John, Batten Carrie, Rajko-Nenow Paulina

Abstract excerpt

Previously, we reported the detection of two novel bluetongue virus (BTV) strains (SPvvvv/02 and SPvvvv/03), possibly representing new BTV genotypes, in a batch of sheeppox vaccine. We developed type-specific RT-qPCR assays (targeting genome segment 2) for these two new BTV strains. The limit of detection of both assays was 10 genome copies/μl and no cross-reactivity with other BTV genotypes was observed. The...

Topics

Animals
Bluetongue
Bluetongue virus
Genotype
Real-Time Polymerase Chain Reaction
Sheep
Vaccines

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Identifiers

PMID: 34536487
DOI: 10.1016/j.jviromet.2021.114288
PMCID: PMC8543067

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