Article
Variation of the permeability of bacteriophage T4: analysis by use of a protein-specific probe for the T4 interior.
Biopolymers - 1 Jan 1991
Griess G A, Khan S A, Serwer P
Abstract excerpt
The permeability of bacteriophage T4 and the change in T4 permeability caused by mutation to osmotic shock resistance are investigated here by quantification of the kinetics with which both a DNA-specific probe (ethidium) and a protein-specific probe [1,1'-bi(4-anilino)naphthalene-5,5'-di-sulfonic acid, or bis-ANS)] bind to T4. In the case of an osmotic shock-resistant mutant, T40s41, both ethidium and bis-ANS...
Topics
- Anilino Naphthalenesulfonates
- Anions
- Buffers
- DNA, Viral
- Electrophoresis, Agar Gel
- Escherichia coli
- Ethidium
- Fluorescent Dyes
- Kinetics
- Light
- Molecular Probes
- Mutation